Low SCF, Good GGA? please check the following:

• Is the GGA fresh and of high quality? Always insure that the GGA solution has not expired and has not been contaminated. Remember, exposure to air can biologically contaminate the GGA solution.
• Has your labware been properly cleaned? Always properly clean and store your bottles, pipettes, tubing, air stones, etc. Sterilization of air stones and tubing is not recommended.
• Has your technique changed? Make sure that your technique is proper and consistent, paying special attention to your pipetting technique.
• Have you checked the water quality? The DI/Distilled water pH should be between 6.5 and 7.5 with a conductivity of less than 1.0 micromhos. The BOD water pH should be 7.2+-0.2 with a conductivity of 125 - 160 micromhos.
• Finally, have you reviewed and are properly following the current PolySeed or PolySeed NX application procedures? A copy of these procedures is provided with each bottle of capsules or liquid and should be reviewed regularly for changes. These procedures can also be downloaded from our website.
  
  Next, after the above has been addressed and problems still exist, do the following:
  
  If your GGA falls into range but you are having problems with low SCF, decrease the PolySeed or PolySeed NX rehydration dilution water by 50-250 mls. This process is approximately linear so if you need to increase the SCF by 20% you should decrease the dilution water by 20%.